Quantitative evaluation of human sperm viability using MTT assay: A laboratory study
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چکیده
منابع مشابه
sperm mtt viability assay: a new method for evaluation of sperm viability
introduction: sperm viability test is important in diagnosis and treatment of male infertility. the common test is e&n; and host which they are based on membrane permeability and integrity. the aim of this study was to evaluate mtt assay as a sperm viability test based on mitochondrial activity. materials and methods: washed sperm samples were co-incubated with mtt in different media and differ...
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The MTT reduction assay depends on the ability of metabolically active cells to reduce the tetrazolium salt (3[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide) to formazan. This study was conducted to examine and validate a simple and less costly MTT test to determine bovine sperm viability and compare the efficiency of this test with a flow cytometer. Fresh ejaculates from eight bull...
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MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored watersoluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studi...
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introduction: the aim of this study was to evaluate the effect of human sperm mtt viability assay on outcome of intracytoplasmic sperm injection. mtt is a tetrazolium salt, routinely used for cell proliferation and cytotoxicity assays. materials and methods: 50μl of processed semen was treated with mtt solution, while the remaining used as the control. meanwhile, 109 donated human oocytes (meta...
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Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...
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ژورنال
عنوان ژورنال: International Journal of Reproductive BioMedicine (IJRM)
سال: 2020
ISSN: 2476-3772,2476-4108
DOI: 10.18502/ijrm.v13i11.7966